recombinant enzymes中文是什么意思

千萬人都在用的超大詞匯詞典翻譯APP

According to the purity and the activity recovery , the recombinant enzyme was well purified by both of these two separation combinations . like natural gloshedobin , the recombinant enzyme exhibited strong esterase activity using tripeptide p - nitroanilide derivatives as substrate , but hydrolyzed n a - p - tosyl - l - arginine methyl ester ( tame ) or n a - benzoyl - l - arginine ethyl ester ( baee ) very weakly
與天然蛇毒類凝血酶一致，當用三肽p山itroanilide衍生物作為底物時，分泌表達的重組大連蛇島蝗蛇毒類凝血酶具有較強的生色底物活性，但用精氨酸甲酯如tame （ na廠tosyl l ar 。
Both the mature genes of gloshedobin and gussurobin were cloned into the vector pet - 32a ( + ) , strain bl21 ( de3 ) , to study their expression in prokaryotic cell . the gene was expressed under t7 promoter with a fusion protein partner of thx . tag and a 6x his . tag at its n - terminal . having been induced by iptg for 4 hours , the recombinant enzyme was examined in the cytoplasm by sds - page analysis
將大連蛇島蝮蛇和長白山白眉蝮蛇毒類凝血酶基因分別克隆到大腸桿菌表達載體pet - 32 ( a ) +中，在t7啟動子下表達出融合蛋白，融合部分為硫氧還蛋白，位于類凝血酶基因上游，并在其n端帶6xhistag標簽以利于表達產(chǎn)物的分離純化，經(jīng)熱激轉(zhuǎn)化至宿主菌bl21 ( de3 )中， iptg誘導斗小時后收獲菌體。

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